TNNT2 is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well
Bovine Troponin T, cardiac muscle (TNNT2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT2 in samples. An antibody specific for TNNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Troponin T, cardiac muscle (TNNT2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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