Vitamin E refers to a group of compounds that include both tocopherols and tocotrienols.[1][2] Of the many different forms of vitamin E, γ-tocopherol is the most common form found in the North American diet.[3] γ-Tocopherol can be found in corn oil, soybean oil, margarine, and dressings.[3][4] α-tocopherol, the most biologically active form of vitamin E, is the second-most common form of vitamin E in the diet. This variant can be found most abundantly in wheat germ oil, sunflower, and safflower oils.[3][5] As a fat-soluble antioxidant, it interrupts the propagation of reactive oxygen species that spread through biological membranes or through a fat when its lipid content undergoes oxidation by reacting with more-reactive lipid radicals to form more stable products.[3][6][1] Regular consumption of more than 1,000 mg (1,500 IU) of tocopherols per day[1] may be expected to cause hypervitaminosis E, with an associated risk of vitamin K deficiency and consequently of bleeding problems.
Bovine Vitamin E (VE) ELISA Kit employs a two-site sandwich ELISA to quantitate VE in samples. An antibody specific for VE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Vitamin E (VE) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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