The protein import machinery of the mitochondrial outer membrane is comprised of a dynamic complex of proteins that mediates translocation of cytosolic precursor proteins into or across the membrane. Several proteins within this complex have been identified, such as Mas20p in yeast and MOM19 in Neurospora crassa .The 145-amino acid human polypeptide shares high similarity with Mas20p and MOM19 within the N-terminal region, but exhibits only weak homology to the tetratricopeptide-repeat B domain that is found in the other 2 proteins. The authors showed that human Mas20p is targeted and inserted into the outer membrane of isolated rat heart mitochondria in the N(in)-to-C(cyto) orientation.
Bovine Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM20 in samples. An antibody specific for TOMM20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM20 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM20 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Mitochondrial import receptor subunit TOM20 homolog (TOMM20) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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