The recombinant mouse IL-4 protein is expressed in E. coli, whose amino acid sequence is derived from mouse IL4 (NP_067258.1), (His23-Ser140) was expressed with a N-terminal Met.
he recombinant mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
Mouse IL-4 protein measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is typically 0.4-2 ng/mL.
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