The recombinant mouse IL-17 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of mouse IL17a (Q62386), (Thr 22-Ala158) was expressed and purified.
The recombinant mouse IL17a consists of 138 amino acids and predicts a molecular mass of 15.5 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
Mouse IL-17 protein measured by its binding ability in a functional ELISA.
Immobilized recombinant mouse IL17a (Cat:51065-MNAE) at 10 μg/mL (100 μl/well) can bind human IL17Ra-Fc2h (Cat:10895-H03H) with a linear range of 16-500 ng/mL.
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