The recombinant mouse IL-6 protein is expressed in E. coli, whose amino acid sequence is derived from mouse IL6 (P08505), (Phe25-Thr211) was expressed and purified.
The recombinant mouse IL6 consists of 188 amino acids and predicts a molecular mass of 21.9 KDa. It migrates as an approximately 22 KDa band in SDS-PAGE under reducing conditions.
Mouse IL-6 protein
1. Measured by its ability to bind biotinylated rat IL6ST-mFch (Cat:80030-R06H) in a functional ELISA.
2. Measured by its ability to bind biotinylated mouse IL6ST-Fc2h (Cat:50135-M03C) in a functional ELISA.
3. Measured by its ability to bind biotinylated mouse IL6ST-His (Cat:50135-M08H) in a functional ELISA.
4. Measured by its ability to bind biotinylated human IL6RB-Fch (Cat:10974-H03H) in a functional ELISA.
5. Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells. The ED50 for this effect is typically 0.4-2 ng/ml.
Abbkine suplies sizes: 5µg, 20µg, 100µg of Mouse IL-6 proteinfor your choice, more detailed information at https://www.abbkine.com/product/mouse-il-6-protein-prp100371.
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