The recombinant human IL-5 protein is expressed in E. coli, whose amino acid sequence is derived from human IL5 (P05113), (Ile20-Ser134) was expressed.
The recombinant human IL5 consists of 115 amino acids and predicts a molecular mass of 13.1 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under non-reducing conditions.
Human IL-5 protein measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/ml.
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