The recombinant human IL-17 protein is expressed in CHO Stable Cells, whose amino acid sequence is derived from mature form of human IL17A(Q16552), (Ile20-Ala155) was expressed with a N-terminal Met.
The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa. It migrates as an approximately 16, 19, and 20 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
Human IL-17 protein
1. Measured by its binding ability in a functional ELISA. Immobilized human IL17A (cat:12047-HNAS) at 10 μg/ml (100 μl/well) can bind human IL17Ra (Cat:10895-H03H), The EC50 of human IL17Ra (Cat:10895-H03H) is 20.0-48.0 ng/ml.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 1-4 ng/mL.
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