The recombinant human IL-17 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human IL17A (Q16552), (Ile20-Ala155) was expressed, with a N-terminal Met.
The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
Human IL-17 protein measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED50 for this effect is typically 15-75 ng/mL.
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