The recombinant human RANKL protein is expressed in Human Cells, whose amino acid sequence is derived from human TNFSF11 (O14788-2), (Gly 63-Asp 244) was expressed.
The recombinant human TNFSF11 consists of 182 amino acids and has a calculated molecular mass of 20.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 27 kDa due to the glycosylation.
Human RANKL protein
1. Immobilized human TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 5.7-13.4 ng/ml.
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL.
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