The recombinant human RANKL protein, Fc Tag is expressed in Human Cells, whose amino acid sequence is derived from human TNFSF11 (AAC51762.1), (Gly63-Asp244) was expressed with the Fc region of mouse IgG1 at the N-terminus.
The recombinant human TNFSF11/mFc comprises 418 amino acids and has a predicted molecular mass of 47.1 kDa. The apparent molecular mass of the monomer is approximately 48-56 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human RANKL protein, Fc Tag
1. Immobilized mouse mFc-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 6.0-14.1 ng/ml.
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 15-45 ng/mL.
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