The recombinant human IL-31 protein, His Tag is expressed in Human Cells, whose amino acid sequence is derived from human IL31 (NP_001014358.1), (Met1-Thr164) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human IL31 consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 31.8 KDa band in SDS-PAGE under reducing conditions.
Human IL-31 protein, His Tag measured by its ability to induce STAT3 activation in U‑87 cells. The ED50 for this effect is 11-45 ng/mL.
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