The recombinant human VEGF165 protein is expressed in Human Cells, whose amino acid sequence is derived from human VEGF165 isoform (P15692-4), (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical.
The recombinant human VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 20 and 22 KDa band in SDS-PAGE under reducing conditions.
Human VEGF165 protein measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 4-16 ng/mL.
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