The recombinant human IL-10 protein is expressed in E. coli, whose amino acid sequence is derived from human IL10 (NP_000563.1), (Ser 19-Asn 178) was expressed.
The recombinant human GADD45G consists of 160 amino acids and predicts a molecular mass of 18.6 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
Human IL-10 protein
1. Immobilized human IL10 at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc (Cat:90125-C02H), The EC50 of Cynomolgus IL10RA-Fc (Cat:90125-C02H) is 0.24-0.56 μg/mL.
2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.2-1.2 ng/mL.
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