The recombinant human IL-10 protein, His Tag is expressed in Human Cells, whose amino acid sequence is derived from human IL10 (NP_000563.1), (Ser 19-Asn 178) was expressed, with a N-terminal polyhistidine tag.
The recombinant human IL10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.
Human IL-10 protein, His Tag
1. Immobilized human IL10-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc (Cat:90125-C02H), The EC50 of Cynomolgus IL10RA-Fc (Cat:90125-C02H) is 0.31-0.71 μg/ml.
2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 5-20 ng/mL.
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