The recombinant human TGFB1 protein is expressed in CHO Stable Cells, whose amino acid sequence is derived from active form of human/rhesus/canine TGFβ1 (NP_000651.3), (Ala 279-Ser 390) was expressed and purified. Human, Rhesus and Canine TGFβ1 sequences are identical.
The recombinant human/rhesus/canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
Human TGFB1 protein Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.04-0.2 ng/mL.
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