The recombinant human IL-35 (IL-12A & IL-27B) protein, Fc Tag is expressed in Human Cells, whose amino acid sequence is derived from human IL35 complex composed of IL27B subunit (NP_005746.2), (Met 1-Lys 229) and the mature form of human IL12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.
The recombinant human IL35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL35/Fc monomer is approximately 90-100 kDa due to glycosylation.
Human IL-35 (IL-12A & IL-27B) protein, Fc Tag measured by its ability to bind biotinylated human IL6RB-Fch (Cat:10974-H03H) in a functional ELISA.
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