The recombinant human EGF protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human EGF (NP_001954.2), (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.
The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.
Human EGF protein measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.) The ED50 for this effect is typically 50-200 pg/mL.
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