The recombinant human IGF1 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human IGF1 isoform 1 (P05019-1), (Gly 49-Ala 118) was expressed and purified.
The recombinant mature form of human IGF1 consisting of 71 amino acids and has a calculated molecular mass of 7.8KDa.
Human IGF1 protein
1. Measured by its ability to bind human IGFBP4-His (Cat:10967-H08H) in a functional ELISA.
2. Measured by its ability to bind mouse IGFBP4-His (Cat:50250-M08H) in a functional ELISA.
3. Measured by its ability to bind Cynomolgus IGFBP1-His (Cat:90085-C08H) in a functional ELISA.
4. Measured by its ability to bind human IGF1R (aa 954-1367)-GST (Cat:10164-H20B1) in a functional ELISA.
5. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.5-2 ng/mL.
6. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is typically 1-4 ng/mL.
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