The recombinant human VEGF-C protein, His Tag is expressed in Human Cells, amino acid sequence derived from mature form of human VEGFC (NP_005420.1) corresponding to amino acid (Thr 103-Arg 227) was expressed with a C-terminal polyhistidine tag.
The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22-24 kDa due to glycosylation.
Human VEGF-C protein, His Tag
1. Measured by its binding ability in a functional ELISA . Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.1-0.5μg/mL.
Abbkine suplies sizes: 5µg, 50µg, 100µg of Human VEGF-C protein, His Tagfor your choice, more detailed information at https://www.abbkine.com/product/human-vegf-c-protein-his-tag-prp100139.
bio-equip.cn