The recombinant human BMP-2 protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human BMP2 (NP_001191.1), (Gln283-Arg396) was expressed. The mature form sequences of human, mouse, rat, rhesus and canine BMP2 are identical.
The recombinant human BMP2 monomer consists of 115 amino acids and has a predicted molecular mass of 13 kDa.
Human BMP-2 protein measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 μg/ml.
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