The recombinant human IL-13 protein is expressed in CHO Stable Cells, whose amino acid sequence is derived from human IL13 (AAK53823.1), (Met 1-Asn 132) was expressed and purified.
The recombinant human IL13 consists of 112 amino acids and migrates as an approximately 12.5 kDa band in SDS-PAGE under reducing conditions as predicted.
Human IL-13 protein measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989, J. Cell Physiol. 140:323.). The ED50 for this effect is typically 0.5-3 ng/mL.
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