The recombinant human IL-33 protein is expressed in E. coli, whose amino acid sequence is derived from C-terminal segment of human IL33 (NP_254274.1), (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.
Human IL-33 protein
1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with anti-CD3. The ED50 for this effect is typically 0.15-0.6 ng/mL.
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