The recombinant human IL-15 protein, His Tag is expressed in E. coli, whose amino acid sequence is derived from human IL15 (NP_000576.1), (Asn18-Ser162) was expressed with a polyhistidine tag at the N-terminus.
The recombinant human IL15 consists of 130 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
Human IL-15 protein, His Tag measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-40 ng/mL.
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