The recombinant human TNF-beta protein is expressed in E. coli, whose amino acid sequence is derived from mature form of human TNFβ (P01374), (Leu 35-Leu 205) was expressed and purified, with an initial Met at the N-terminus.
The recombinant human TNFβ comprises 172 amino acids and predicts a molecular mass of 18.8 kDa as estimated in SDS-PAGE under reducing conditions.
Human TNF-beta protein measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.1-0.5 ng/ml.
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