The recombinant human IL-1 beta protein (pro form), His Tag is expressed in E. coli, whose amino acid sequence is derived from pro form of human IL1β (NP_000567.1), (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus.
The recombinant pro form of human IL1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions.
Human IL-1 beta protein (pro form), His Tag
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 μg/mL.
Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.