The recombinant human BAFF protein is expressed in Human Cells, whose amino acid sequence is derived from soluble form of human BAFF (Q9Y275-1), (Ala 134-Leu 285) was expressed and purified.
The recombinant human BAFF consists of 152 amino acids and has a predicted molecular mass of 17 kDa. In SDS-PAGE under reducing conditions, rhBAFF is migrates as an approximately 19 kDa band.
Human BAFF protein measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is typically 0.4-2 ng/mL in the presence of goat anti-mouse IgM μ chain.
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