There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIM domain kinase 2 encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and LIM domain kinase 2, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.
The Abbkine LIMK-2 (phospho Thr505) Rabbit Polyclonal Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and detects endogenous levels of LIMK-2 protein only when phosphorylated at T505. The antibody has been validaed in WB, IHC-P, IF, ELISA and reacts with Human, Mouse, Rat samples.
Abbkine supplies below price/size: $100/30μl, $220/100μl, $360/200μl of Rabbit Anti-LIMK-2 (phospho Thr505) Polyclonal Antibody at https://www.abbkine.com/product/limk-2-phospho-thr505-polyclonal-antibody-abp50354.
bio-equip.cn
中文
