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LAMBDA MIINFOR Fermentor:

The LAMBDA MINIFOR bench-top laboratory fermenter and bioreactor was developed as a result of the need to construct a small laboratory fermentor for volumes from 35 ml to over 6 liters. Based on long personal practical experience of fermentations we wanted to create a fermentor, which was easy to use and with the capacity to measure and control all the important parameters of the biological culture.

The fermentor had to take up minimum space on the bench but with good access to all parts. Several fermentors should when placed side by side be suitable for the optimization of the parameters of growth of culture or optimization of bio-transformations etc.

To keep the cost of the MINIFOR fermentor-bioreactor low, without compromising quality, several new ideas and innovations have been introduced:

Instead of a fermentor flask with a stainless steel cover, which is expensive, we use whole glass vessels with threaded fittings. They have been used for many years in cell culture and are proved to maintain perfect sterility. Thanks to this concept, the MINIFOR fermenter-bioreactor is set up in shortest possible time.
Instead of a traditional propeller agitator, which requires an expensive motor and magnetic coupling, we have introduced a new up-and-down agitation. A motor together with an inexpensive membrane perfectly assure sterility and produce an efficient mixing without formation of a vortex (no baffles needed). At the same time this type of mixing is gentler on cells and produces less foam. Novel biomimicking “fish-tail” stirring discs offer maximum mixing efficiency without cutting edges.
The culture is heated by heat radiation produced in a parabolic radiator with a gold reflector placed under the fermentation vessel. The heat is absorbed gently in the culture similarly to the sun heating water. There is no overheating of the culture at any volume and expensive double-wall vessels with thermostatic baths are eliminated. At the same time tubing and cables disappear making the fermentor less complex.
As far as possible expensive pieces of equipment have been replaced by new high performance plastics.
By using modern microprocessors it has been possible to place all the electronics in the front part of the instrument. This makes the fermentorunbelievably compact and eliminates the casing tower. Despite its small size six parameters are measured and controlled in the basic configuration of the MINIFOR.

Each fermentor should be able to work independently or be connected to a PC for advanced regulation and extensive data treatment.

In Chinese:-

實驗室級發酵槽 FERMENTOR MINIFOR
1. 微處理機 (Microprocessor) 控制
2. 槽體 : Pyrex 玻璃材質, 具 5 (標準) -8 組分支槽口, 容積 1(標準) -3 公升
3. 電源 : 90-245 VAC/50-60Hz, 符合 CE 標準
4. 尺寸 : 22(W)×40(D)×38(H)cm
5. 溫度控制 : 以特殊之 100W 具鍍金反射鏡之紅外線加熱
  • 調整 : 室溫 +5°C - 70°C
  • 測量 : 0°C - 99.9°C
  • 準確度 : ±0.2°C (0-60°C)
  • 溫度探針 : Pt100 結合於 pH 電極上
6. pH 控制 : 可滅菌式 pH 電極 (pH 0-13), 具自動溫度補償, Variopin 接頭及兩點校正
  • 解析度 : 0.01pH
  • 準確度 : 0.02pH
7. 溶氧控制 : 可滅菌之 Clark 式溶氧電極, 具快速反應及強化之 Teflon 薄膜
  • 測量範圍 : 0-10mg oxygen/l, 最小刻度 0.1mg/l
8. 空氣流量控制 : 0-5 l/min, 最小刻度 0.01 l/min 之流量計控制。線性 : 3%, 再現性 : 0.5%
  • 控制 : 利用記憶金屬之比例閥
9. 攪動器 : 40W 磁式震盪器, 0-20Hz, 可具 1 或多組攪拌碟, 採震盪式攪拌, 無旋渦及發泡產生。同時, 進氣之氣泡口結合於攪拌碟之下方, 充分將氣體均勻分佈於槽內
10. 取樣口 : 最多可接四組 Luer-Lock 之針頭, 用於添加或取樣
11. 幫浦 : 最多可接四組獨立之幫浦, 可調整速度變化 0-100%
12. 其他選項 : Foam control、CO2 溶解量、Optical density 及 conductivity 等
13. 可外接 PC (RS485 介面) 控制

Technical description	Advanced Kit	Start-up Kit
Reactor scale	Laboratory bench-top fermentor and bioreactor
Reactor design	Stirred Tank
Operation mode	batch, fed-batch, continuous, (perfusion*)
Parameter control	stirrer speed, temperature, pH, Dissolved Oxygen, airflow, free parameter, level control for continuous mode.
* Optional Parameter control *	weight control for continuous mode, antifoam, REDOX, LUMO (light)
Microprocessor With internal software, display and keypad	yes	yes
Agitation Automatic stirrer controller 0, 0.1, 0.2, …., 19.9, 20 Hz min. 0 Hz ( = 0 rpm) max. 20 Hz ( = 1200 rpm)	yes	yes
Measurement whole range 0 - 20 Hz	yes	yes
Temperature Automatic temperature control from 5 °C over RT to 70 °C	yes	yes
Measurement with Pt100 from 0 to 99.9 °C in 0.1 °C steps	yes	yes
IR (infrared) heater	yes	yes
Cooling loop	yes	yes
pH Automatic pH controller 0 - 13	yes	yes
Measurement with pH probe 0 -13	yes	yes
Acid pump (PRECIFLOW)	yes	optional
Base pump (PRECIFLOW)	yes	optional
Holders and rods for the pumps	yes	optional
Storage vessels and lines for acid and for base	yes	optional
DO (pO2) Automatic DO controller (proportional) 0 - 25 mg Oxygen/L (in 0.1 mg steps)	yes	yes
Measurement with DO probe 0 - 25 mg Oxygen/L (in 0.1 mg steps)	yes	optional
Airflow Automatic aeration controller (proportional) 0 - 5 L/min in 0.01 L/min steps	yes	yes
Measurement with internal Massflow 0 - 5 L/min in 0.01 L/min steps	yes	yes
Free Parameter Automatic controller, socket, display	yes	yes
AlertsFor high and low values of each parameter	yes	yes
Overpressure valve	yes	yes
Sampling device	yes	optional
Cooling device for outlet gas	yes	yes
PC-software (FNet, SIAM), Laptop	optional	optional
Feed and harvest pump: PRECIFLOW / MULTIFLOW / HiFLOW / MAXIFLOW / VIT-FIT / VIT-FIT HP, pump INTEGRATORS	optional	optional
WEIGHING MODULE for continuous mode	optional	optional
automatic Antifoam-system (ANTIFO & DOZITO)	optional	optional
O2-enrichment / 4-gas-mix: controller and massflows MASSFLOW 500 /MASSFLOW 5000	optional	optional
Air compressor AEROSILENTO	optional	optional
REDOX probe, controller, pump / massflow	optional	optional
PELTIER COOLER for medium / off-gas	optional	optional
LUMO light and controller for PBR	optional	optional
Operation manual and video	yes	yes
Warranty	2 years

Accessories：

Automatic gas mixing module MINI-4-gas

Fermentation Software FNet

MASSFLOW gas flow measurement and controller

Scale module for continuous culture (chemostat)

Red-Ox potential (ORP) measurement

Antifoam control for protein-rich media

Optical Density (OD) measurement

Parallel cultivation/processes

Feed pump for fed-batch cultures