Contact: reagenllc@gmail.com / anna@reagen.us
REAGEN™ Aflatoxin B1 ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals, feed, milk , cookies, oils, peanuts .The unique features of the kit are:
- High recovery (75-125%),rapid(10-40minutes), cost-effective extraction methods.
- High sensitivity (0.02 ng/g or ppb).
- High reproducibility.
- A quick ELISA assay (only 1 hour regardless of number of samples).
Procedure Overview
REAGEN™ The method is based on a competitive colorimetric ELISA assay. The Aflatoxin B1-BSA has been coated in the plate wells. During the analysis, sample and Aflatoxin B1 antibody (Antibody#1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Aflatoxin B1 residue is present in the sample, it will compete with the Aflatoxin B1 on the plate wells for the Aflatoxin B1 antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Aflatoxin B1 residue concentration in the sample.
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