OmniPCR (OnePCR Ultra) is a ready-to-use combined solution containing the Thermostable DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. Directly add primers, template, and water to execute the polymerase chain reaction (PCR). The Taq DNA polymerase exhibits 5′-exonuclease activity and after carrying DNA electrophoresis, the OmniPCR (OnePCR Ultra) fluorescence dye is directly detected on BluPAD LED transilluminator or other compatible transilluminator. The supermix is provided at 2x concentration and used at 1X concentration for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each, 100 reactions per kit.
Application
→ No post-staining processing of DNA required.
→ Direct loading onto the agarose gel for electrophoresis.
→ Sensitivity – High degree of sensitivity equivalent to ethidium bromide.
→ Speed – Benefit for quick set up of PCR reaction such as colony PCR when screening for positive clones.
→ Compatibility – Use blue light or UV to detect the signal.
Storage
Stable for up to 6 months at 4°C.
Stable for up to 24 months at -20°C.
Note: OmniPCR (OnePCR Ultra) is light sensitive and should be stored and protected from light.
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