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Mycobacteria L-J Culture Medium(Culture Method) / Reagents test kits
Mycobacteria L-J Culture Medium(Culture Method) / Reagents test kits
Place of Origin:
China
Brand:
encode
Model:
M0007
Price:
USD1.1-USD1.3
Hits:
889 
Updated:
5/29/2015
  • Product Detail
  • Company Profile

     

    INTENDED USE

     
    This product is used for drug susceptibility test of mycobacteria.
     

    PRINCIPLE

     
    The product is to test drug susceptibility of mycobacteria with ratio method. The medium contains rich nutrients and different drugs with constant concentration. In the medium, inorganic salts, carbon source, nitrogen source, and water work to stimulate the mycobacterium growth, whereas the different drugs inhibit the mycobacterium growth.
    Pick mycobacteria, isolated with liquid or solid medium to adjust to a certain concentration, inoculate respectively to different drug susceptibility test L-J media and control media, incubate at 37°C for 4 week, and finally evaluate the drug susceptibility after observing the growth of mycobacteria on drug susceptibility test medium and control medium.
     

    COMPONENTS

     
    Slant medium Package insert. Tubes
    Main ingredients in medium: anti-mycobacterial drugs, potassium dihydrogen phosphate, magnesium sulfate, magnesium citrate, sodium glutamate, glycerol, malachite green and fresh eggs, etc.
    Detailed as follows:
    drugs for susceptibility test
    drugs for susceptibility test
    Isoniazid(INH)
    Ofloxacin(OFLX)
    Rifampicin(RFP)
    Kanamycin(KAN)
    Ethambutol(EMB)
    Streptomycin(SM)
    Rifapentine(RFPT)
    Pyrazinamide(PZA)
    Paraaminosalicylic acid(PAS)
    Prothionamide(PTA)
    Amikacin(AMK)
    2-Thiophenecarboxylicacid, hydrazide(TCH)﹡
    P-nitrobenzoic acid(PNB)﹡
     
    *:for differentiation between Mycobacterium tuberculosis and Mycobacterium bovis
     

    STORAGE AND VALIDITY

     
    ·     Sealed and stored at 2-8°C in a cool and dry place.
    ·     The validity duration is 6 months
     

    SPECIMEN COLLECTION

     
    ·     The clinically freshly isolated colony culture (initially within 2 weeks), which does not require subculturing, is qualified for susceptibility test.
    ·     Over 2 weeks isolated colony and stored colony should be subcultured on L-J culture medium. Collect the grown colony after 2~3 weeks.
    ·     Mycobacteria obtained from isolation on logarithmic growing stage, should be diluted to 10-2 mg/ml and 10-4 mg/ml by grinding.
     
     

    PROCEDURE

     
    1. Calibration of concentration and preparation of suspension inoculation.
    ·     Specimen from liquid medium: centrifuge the liquid specimen at 4000g for 15 minutes, then get the sediment, which is diluted with 0.5% sterile saline, and finally obtain mycobacterial suspension with turbidity of 1mg/ml.
    ·     Specimen from solid medium: pick mycobacterial colonies, which are diluted with 0.5% sterile saline, then obtain mycobacterial suspension with turbidity of 1mg/ml.
    ·     Suspension diluted to inoculation concentration: The suspension by turbidimetry is diluted with 0.5% sterile saline.
    2. Inoculation : With inoculation concentration10 -2 mg/ml and 10-4 mg/ml ready, transfer 0.01ml with inoculating loop, then inoculate respectively to drug susceptibility medium and control medium
    3. Culture: Tilt medium after inoculating and keep at 37°C to culture for 24-48h with cap loose, screw back the cap to culture for another 4 week; If mycobacteria grow well in the control tube, drug susceptibility can be reported, if no mycobacteria grows, repeat the test.
     

    REFERNCE VALUE

     
    ·     Susceptibility: If there are mycobacteria growing in the control tube, drug susceptibility can be reported.
    ·     Negative: Detection on negative control material.
    bio-equip.cn
    Zhuhai Encode Medical Engineering Co.,Ltd (herinafter referred to as Encode) was established in 1994, as one of the earliest
    and largest manufacturers of in-vitro diagnostic field in China. Encode specializes in R&D, production and distribution of in-vitro diagnostic products. As a hi-tech and innovative enterprise, we have researched and developed series of microbiological diagnostic productds and immunology diagnostic rapid tests, which are used for diagnosis of infection disease, cardiac markers, tumor markers, fertility test, durgs of abuse and sexual transmitted disease.

    Encode occupies a land of 15,000 m2 in Zhuhai. There are 2,000 GMP standard clean workshops of microbiology diagnostic products, colloidal gold diagnostic products and molecular with automatic manufacturing lines and passed the quality management system evaluation by SFDA and GDFDA.

    Encode has established extensive cooperation with research centers in universtities, such as Lanzhou University, South China University of Technology, Jinan University and Shanghai Plumonary Hospital, etc. Whith strong R&D and technological innovation capability. Encode ranks leading level in terms of IVD industries field in China. We have also obtained ISO 13485:2003, ISO 9001:2001, CE and have been granted the title of national hi-tech enterprise.

    Today, we provide medical products and health care solution to clients not only in China, but also worldwide, such as Europe, Africa, Middle East, South East, Asia etc.

    With the experience and dedication of the scientific staffs, Zhuhai Encode Medical Engineering Co.,Ltd will continue our endeavor to provide professional in-vitro diagnostic products and health care solutions to the worldwide and make our contributions to the career of human health.
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