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Comet Assay Tanks
Comet Assay Tanks
Place of Origin:
United Kingdom
Brand:
cleaver
Model:
CSL-COM
Supplier:
CLEAVER SCIENTIFIC LTD
Price:
Hits:
1508 
Updated:
6/11/2016
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    Comet Assay Tanks

    ·For Single Cell Gel Electrophoresis

    ·Minimise exposure to light and reduce background DNA damage

    ·High efficiency cooling for enhanced resolution 

    Product Description

    Cleaver Scientific Comet Assay Tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantifation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using Comet Assay scoring software.

    Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.

    Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a comet as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.

    Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.

     

    Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.

     

    Variants:

    ·CSL-COM10 – Comet Assay Tank for 10 Slides

    ·CSL-COM20 – Comet Assay Tank for 20 Slides

    ·CSL-COM40 – Comet Assay Tank for 40 Slides

     

    ·CSL-COM80 – Comet Assay Tank for 80 Slides

     
    bio-equip.cn
    CSL is a privately owned company set up in 2004 with the sole aim to become one of the world’s leading suppliers of electrophoresis and related products. The management team have a wealth of experience in the design, manufacture and support of these products and we are an ISO9001:2000 approved company.

    We pride ourselves on our approach to working closely with our customers to make sure they get the quality & service they require and with this in mind our mission statement is as follows:

    Our Mission Statement

    CLEAVER SCIENTIFIC LTD is an innovative leader in the design and manufacturing of electrophoresis equipment and related products for the life science market and general plastic fabrication and assembly.We are committed to TOTAL CUSTOMER SATISFACTION through continuous improvement in our people, products and processes.

    To meet these aims, we will manage our business under the principles set out in ISO9001 and ISO14001 to which we are totally committed. This commitment also extends to not only meeting customer, regulatory and other requirements, but to continually improve the effectiveness of our management systems. We are also committed to the prevention of pollution, incidents and accidents and errors within our management systems.

    Our business plan provides the basis for setting goals, objectives and measurements which are communicated throughout the company. These objectives are continually monitored to identify any opportunities for improvement which will benefit Cleaver Scientific Ltd., our customers, and other interested parties.

    All employees are aware of their contribution to the future of the Company, and their conformance with its requirements is recognised as playing a significant part in a successful, long term and fruitful partnership.
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