PrimeTime qPCR DNA probes are non-extendable oligonucleotides labeled with a 5’ fluorescent reporter and a 3’ quencher dye licensed for use in 5’ nuclease qPCR assays (with companion dye and quencher licenses).
PrimeTime® qPCR Probes
- Traditional dual-labeled probes are available with a wide variety of dyes and quenchers.
- Choose IDT proprietary ZEN™ Double-Quenched Probes for superior performance compared to traditional dual-labeled probes.
- Ultra-small-scale Mini Probes delivered at 0.5 nmole allow researchers to conduct initial screening at low cost.
- Eco Probes are delivered at 2.5 nmoles normalized yield, sufficient for ~500 reactions for gene expression analysis.
- Express Probes are ready for shipment in one business day.
- The ZEN™ quencher enables the production of Double-Quenched Probes that provide less background and increased signal!
- Double-Quenched Probes are available with 5’ FAM, TET, HEX, MAX, or JOE fluorophores, a 3’ IBFQ quencher, and an internal ZEN™ quencher.
- ZEN™ Double-Quenched Probes display consistently earlier Cq values and improved precision when compared to traditional probes.
For more information download the ZEN Double-Quenched Probe Overview.
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About IDT
Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.
Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:
Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.
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