xGen® Blocking Oligos,idt_specification/price/image

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Enhance the Performance of Target Capture for Next Generation Sequencing

xGen® Blocking Oligos bind to platform adapter sequences on a designated strand (usually the inverse of the synthetic adapter) to help prevent non-specific binding, improve the number of reads on-target, and increase the depth of enrichment.

Benefits

Increase sample throughput by effectively blocking multiple pooled library adapters with a single blocking oligo
Easy and flexible ordering of predesigned sequences that correspond to commonly available adapter library preparation kits

Features

A single xGen® Universal Blocking Oligo effectively blocks multiple barcoded adapters
Easily order from predesigned xGen Standard Blocking Oligos for low throughput target capture

Prevent Adapter Cross-Hybridization

Adapter sequences specific to the platform being used for next generation sequencing platform are ligated to all library fragments, both on-target and off-target, before enrichment. These adapter sequences can hybridize with each other during enrichment, creating a "daisy-chain" effect in which off-target fragments are captured along with on-target fragments.

xGen® Blocking Oligos bind to platform adapter sequences on a designated strand (usually the inverse of the synthetic adapter) to help prevent non-specific binding (Figure 1). HPLC purification of your blocking oligos can improve binding to adapter sequences. Introducing end-terminating modifications, such as a 3' C3 spacer, helps to inhibit spurious amplification of blocking oligos.

Enhance Capture Performance by Blocking Platform Adapters

FIGURE 1. BLOCKING OLIGOS INCREASE ON-TARGET READ PERCENTAGE. Several published articles have shown markedly higher on-target percentage by adding oligo blockers. For example, Blumenstiel et al. [1] increased on-target reads from 35% to 59% by including blocking oligos before target capture; Hodges et al. [2] achieved even better performance with blocking oligos, improving their on target reads from 32% before blocking oligos to 64% after the addition of blocking oligos. This approximately two-fold increase in on-target reads allows for multiplexing of a greater number of samples and increases read depth for easier identification of rare alleles.

High and Low Throughput Options with Excellent Peformance

References

Blumenstiel B, Cibulskis K, et al. (2010) Targeted exon sequencing by in-solution hybrid selection. Curr Protoc Hum Genet, Chapter 18:Unit 18.4.
Hodges E, Rooks M, et al. (2009) Hybrid selection of discrete genomic intervals on custom-designed microarrays for massively parallel sequencing. Nat Protoc 4(6):960–974.