SURVEYOR® Mutation Detection Kits provide a simple and robust method to detect mutations and polymorphisms in DNA. The key component of the kits is SURVEYOR Nuclease, a member of the CEL family of mismatch-specific nucleases derived from celery. SURVEYOR Nuclease recognizes and cleaves mismatches due to the presence of single nucleotide polymorphisms (SNPs) or small insertions or deletions.
Benefits
- Detect base-substitution and insertion/deletion mismatches.
- In a single reaction, identify mutations in DNA fragments up to 3.5 kb.
- Quickly determine the number of mutations by observing the number of cleavage products.
- Detect 1 mutant allele out of 32 alleles in pooled samples.
- Use with various analysis platforms.
Designed to detect mutations in DNA derived from a variety of organisms including bacteria, fungi, plants, and animals, SURVEYOR® Mutation Detection Kits allow analysis by:
·Standard gel electrophoresis
·WAVE® and WAVE HS Systems
SURVEYOR Nuclease is provided as a standardized, quality-controlled enzyme formulation in a ready-to-use kit that can be used without further batch-to-batch optimization. All components except the thermostable DNA polymerase and PCR products are provided. Reaction buffers and positive controls included in the kit allow users to set up and monitor the assays to obtain reproducible results. In-depth SURVEYOR Mutation Detection Kit User Guides provide detailed instructions and troubleshooting.
SURVEYOR® Mutation Detection Kit for Standard Gel Electrophoresis
The SURVEYOR® Mutation Detection Kit for Standard Gel Electrophoresis has been designed to cleave unlabeled DNA fragments at mismatched sites for subsequent analysis by agarose gel electrophoresis or polyacrylamide gel electrophoresis (PAGE). DNA of 200 to 4,000 bp can be analyzed using manual agarose gel electrophoresis while smaller fragments (<1,000 bp) can be analyzed using manual polyacrylamide gel electrophoresis (PAGE).
SURVEYOR® Mutation Detection Kit for WAVE and WAVE HS Systems
The SURVEYOR® Mutation Detection Kit for WAVE and WAVE HS Systems has been designed to cleave DNA fragments at mismatched sites for subsequent analysis by ion-pairing reverse-phase HPLC using the WAVE and WAVE HS Systems.
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