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KOD+ DNA Polymerase
KOD+ DNA Polymerase
Place of Origin:
China
Brand:
Shanghai ShineGene Molecular Biotech,Inc.
Model:
Supplier:
Shanghai ShineGene Molecular Biotech,Inc.
Price:
Hits:
3307 
Updated:
3/21/2020
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    Catalog #

    Pack size

    Price(€)

    ZP02033

    200U

    55.00

    ZP02034

    500U

    120.00


    Description:
    DNA polymerase from Thermococcus kodakaraensis KOD is one of the most efficient thermostable PCR enzymes exhibiting higher accuracy and elongation velocity than any other commercially available DNA polymerase.The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5''---->3'' direction. The KOD DNA Polymerase also exhibits 3''--->5''exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5''--->3'' exonuclease activity.
    Concentration:5U/ul

    Source:
    KOD DNA Polymerase (native) - Thermococcus kodakaraensis cells.KOD DNA Polymerase (recombinant) - E.coli cells with a cloned pol gene from Thermococcus kodakaraensis.
    10X Reaction Buffer with MgCl2:
    1 ml 10X Buffer : 1.2 M Tris-HCl, 100 mM KCl, 60 mM (NH4)2SO4, 25 mM MgCl2, 1% Triton X-100, 0.01% BSA, pH8.0 .
    Features:

    High fidelity than other normal DNA polymerase
    Higher processivity.
    Higher yield in short time

    Applications:
    All PCR* amplification which demands high fidelity.
    Primer extension.
    High fidelity PCR for cloning into blunt-ended vectors.
    Site-directed mutagenesis.

    Unit Definition:
    One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol dNTP into acid insoluble form in 30 minutes at 75°C in a reaction containing 20 mM Tris-HCl (pH7.5 at 25°C), 8 mM MgCl2, 0.5 mM DTT, 50ug/ml BSA, 150 uM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]-dTTP) and 150 ug/ml activated calf thymus DNA.

    Quality Control Tests:
    PCR (activity), SDS-PAGE (purity), endonuclease/nickase.

    Storage:
    5U/ul in 50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.1% Nonidet P-40, 0.1% Tween-20, pH8.0
    bio-equip.cn
    ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

    http://www.synthesisgene.com
    http://www.shinegene.org.cn/englsih
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