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PowerQ® Taq DNA Polymerase for qPCR
PowerQ® Taq DNA Polymerase for qPCR
Place of Origin:
China
Brand:
ShineGene
Model:
Supplier:
Shanghai ShineGene Molecular Biotech,Inc.
Price:
Hits:
2683 
Updated:
3/21/2020
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    Catalog #

    Pack size

    Price(€)

    ZP00102

    1000U

    26.40

    ZP00103

    5000U

    106.40


    Storage condition: The undiluted enzyme solution is stable when stored at -20°C.

    Storage and dilution buffer: 20mM Tris-HCl; 1mM dithiothreitol; 0.1mM EDTA; 0.1M KCl; Nonidet P40, 0.5%(v/v); Tween 20, 0.5%(v/v); glycerol, 50%(v/v); pH 8.0 (4°C).

    Concentration:5U/ul

    Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10mmols of dNTP into an acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl, (pH 9.0 at 25°C), 50mM NaCl, 10Mm MgCl2, 200uM dATP, dCTP, dGTP and radiolabelled dTTP, and 12.5ug activated calf thymus DNA in a 50ul reaction.

    10XReaction buffer(without MgCl2): 100mM Tris-HCl; 500mM KCl; pH 9.0 (20°C). Buffer is optimized for use with 0.2mM for each of dNTPs. A tube of 25mM MgCl2 is supplied separately.

    Note: It is recommended to add dNTPs to this incubation mixture shortly before use. This is to prevent decomposition of the deoxynucleoside thiphosphate that occurs during Prolonged storage at the alkaline pH values required for optimal enzyme activity.

    Quality control

    Each lot of Taq DNA Polymerase is tested for activity in PCR and efficient incorporation of digoxigenin-11-dUTP, and in DNA sequencing of M13mp18ssDNA. A minimum of 250 bases must be clearly legible in the sequencing gel. Each lot of Taq DNA ploymerase is tested for contaminating activities as stated below.

    Test buffer: 10mM Tris-HCl; 1.5mM MgCl2; 50mM KCl; pH 9.0 (20°C). 0.1mg/ml gelatin.

    Absence of endonucleases: 1ul lambda DNA is incubated with Taq Dna polymerase in 50ul test buffer with a paraffin oil overlay at 65°C for 16 hours. The amount of enzyme showing no degradation of the lambda DNA is ststed under "Endol".
    bio-equip.cn
    ShineGene Bio-Technologies, Inc., located in Shanghai, P.R. China, is a bio-company specialized in providing bio- technical services including Gene Synthesis,Peptide Synthesis,Real Time PCR, Fluorogenic Probe Design,Fluorogenic Probe Label,HRP Enzyme Stabilizer,X-gal,IPTG,ProClin300etc. and supplying reagents such as Hotstart Taq polymerase, dNTPs, qPCR mastermix,one step qPCR kits,First Strand cDNA Synthesis Kits,etc. ShineGene also undertake some OEM or OED orders as well as production task.ShineGene works at the cutting edge of development in PCR, quantitative real-time PCR, transposon and RNA technologies. ShineGenes mission is to provide new tools of excellent quality and extreme purity to the scientific community. Therefore research and development is a continuous process in our company.

    http://www.synthesisgene.com
    http://www.shinegene.org.cn/englsih
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