Description:
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid. Two molecules of BCA chelate with one cuprous ion forming purple-coloured product. The BCA/copper complex is water-soluble and exhibits a strong absorbance at 562nm. Protein concentration can be determined based on absorbance because of the linear relation between them.
Features:
Faster and easier than the Lowry method
Sensitive, The minimum detection level of 25μg/ml.
Compatible with most detergents(SDS/TritonX-100/ Tween20/Tween60/Tween80 up to 5%).
Good linear range from 20-2000μg/ml.
Coefficient of variation lower than Bradford.
bio-equip.cn
BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/
Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.
Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.
Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.
Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.
samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.