pUM-T Fast Cloning Kit
| Cat.# |
|
Preps
|
Price(USD)
|
|
DP6701
|
|
20
|
86
|
|
DP6702
|
60
|
152
|
|
DP6703
|
|
20
|
86
|
|
DP6704
|
60
|
152
|
Description
pUM-T vector is designed for PCR cloning by BioTeke. pUM-T vector derived from pUC18 provides 3’ deoxythymidine (T) by adding certain sequence and specific procedure. Taq polymerase has a non template-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3’ ends of PCR products. This allows PCR inserts to ligate efficiently with the vector. The insert does not change lac Z reading frame, so positive clones can be picked by blue/white screening. In Addition, pUM-T vector contains pUC18 multiple cloning sites. The insert can be confirmed by double digestion or sequenced using M13 universal primers.
Features
* Specific rapid ligation buffer ensures ligation in 10-15min.
* Optimized vector sequence increases transformation efficiency.
* Specific T vector storage buffer keeps T vector more stable.
* pUM-T contains LacZ for white/blue screening.
* pUM-T contains pUC18 mutiple cloning sits for confirmation.
bio-equip.cn
BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/
Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.
Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.
Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.
Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.
samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.
Our R
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