M13 Phage Single Strand DNA Rapid Extraction kit (Spin-column)
SKU |
Product |
Preps |
Price(USD) |
DP2301 |
M13 Phage Single Strand DNA Rapid Extraction kit (Spin-column) |
50 |
67 |
DP2302 |
100 |
115 |
Description |
This kit provides a rapid, reliable and high-throughput method for the purification of high-quality genomic DNA from M13 Phage |
Primary principle
The infected bacterial culture firstly is centrifuged to pellet the bacterial cell, and then high-salt buffer is added to the supernatant and single-strand phage DNA is selectively absorbed to silica membrane. The sample is then applied to a spin column and centrifuged. DNA binds to the membrane while contaminants such as proteins and polysaccharides are efficiently removed by two wash steps. Pure single-strand phage DNA is eluted in a small volume of low-salt buffer or water.
Feature and benefits
High yield, generally purify up to 3μg of single-stranded DNA from 800mL of phage supernatant.
Rapid, single sample DNA isolation under 10 min
High purity, Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9 which is suitable for sequencing and other biological applications.
bio-equip.cn
BIOTEKE is a leading biotechnology company in China since 2005,
Our products nucleic acid extraction/purification as well as related essential lab instruments.
Please check our website here: http://english.bioteke.cn/
Our main product portfolio includes but not limits the following items,
Reagents/kits for molecular biological research uses,
DNA/RNA/plasmid extraction kits, PCR/RT-PCR/qPCR products, DNA/RNA stains/markers.
Automated Nucleic Acid Extraction systems, together with it's magnetic beeds kits;
For extracting DNA/RNA from various samples(cfDNA, blood, tissue/cells, plants, plasmid, etc) ,
32 samples or 96 samples in one time in 30min,
The cost for one samples is less than 4USD for our clients.
Micro Spectrophotometer ND5000,
0.5-2uL sample is needed, 4s for 1 test, to test the concentration of DNA/RNA/proteins.
Storage tubes for blood RNA, cfDNA, Urine DNA, swab/saliva DNA, stool DNA samples.
samples can be transported for more than one week, or for long term storage in low temprature.
widely used in clinical sample collections.Trial samples are available.
Our R