Description:
RNzol Reagent is a ready-to-use reagent for the extraction of total RNA from samples such as cells, tissues and plants. The reagent, a mono-phasic solution of phenol and guanidine thiocyanate, is an improvement to the onestep RNA isolation method developed by Chomczynski and Sacchi. During sample homogenization or lysis, RNzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Add chloroform followed by centrifugation, separate the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropanol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation.

Total RNA extacted by RNzol Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning. For use in the polymerase chain reaction (PCR), treatment of the extracted RNA with amplification grade DNase I is recommended when the two primers lie within a single exon.

RNzol Reagent facilitates isolation of a variety of RNA species of large or small molecular size. For example, RNA isolated from rat liver, electrophoresed on an agarose gel, and stained with ethidium bromide, shows discrete bands of high molecular weight RNA between 7 kb and 15 kb in size, (composed of mRNA’s and hnRNA’s) two predominant ribosomal RNA bands at ~5 kb (28S) and at ~2 kb (18S), and low molecular weight RNA between 0.1 and 0. 3 kb (tRNA, 5S). The isolated RNA has an A260/A280 ratio≥ 1.8 when diluted into TE.

Specifications:
Mono-phase total RNA extraction reagent;
Extract RNA from variety organism samples;
Inhibit RNase activity;
Remove DNA and proteins effectively.
Applications:
Tissue(50-100mg/ml): varieties fresh or freeze tisse
of human, animal, plant
Cell(5~10×10⁶ /ml) : eukaryote cell, bacteria,
yeast
Liquid(100μl/ml): samples of whole blood,
humour, urine, viruses,etc

Storage:
4℃one year in the dark.
Expected yields of RNA per mg of tissue or 1×10⁶ cultured cells
Liver and spleen, 6-10μg
Kidney, 3-4μg
Skeletal muscles and brain, 1-1.5μg
Placenta, 1-4μg
Epithelial cells (1 x 10⁶ cultured cells), 8-15μg
Fibroblasts, (1 x10⁶ cultured cells) 5-7μg

1. Invitrogen TRIzol
2. Ambion
3. BioTeke RNAclean
4. BioTeke RNzol
5. BioTeke RNApure
(RNA extracted from plant leaves with different reagents in 1% agrose gel)