Human Adipose-Derived Stem Cell Serum-free Medium (HADSC-SFM)

★ Technical Manual

      Human Adipose-Derived Stem Cell Serum-free Medium (HADSC-SFM)

★ Catalog NO: BW110021

★ Components

         490 ml of Basal Medium

              5 ml of Human Adipose-Derived Stem Cell Supplement (HADSCS)  

              5 ml of penicillin/streptomycin solution (P/S)

★ Product Description

      Human Adipose-Derived Stem Cell Serum-free Medium (HADSC-SFM) contains defined components including essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and other supplements. The product is suitable for culturing human adipose-derived stem cells. The product is strictly aseptic without any contamination of virus or mycoplasma, and exhibits stable performance. By using this medium, human adipose-derived stem cells can be quickly expanded with minimal differentiation.

★ Instruction for Use

    It is strongly recommended that cell plates should be precoated with CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).

   1. Dilute CELLstart™1:50 (Cat. No. A10142-01, GIBCO) in Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (Cat. No. A12858, GIBCO).

   2. Add diluted CELLstart™ to culture container at a final volume per surface area of 0.078 mL/cm². Refer to table for respective culture container: 

     Culture Container                   Surface Area (cm²)         Volume of 1:50 Diluted CELLstart

             6-well plate                             9.6                                                   750ul per well

            12-well plate                            3.2                                                   250ul per well

            24-well plate                            2.0                                                   160ul per well

3. Incubate in a 37°C, 5% CO2 incubator for 2 hours. It is recommended to coat culture containers the day of use or the day before. If precoating the day before, the culture container must be stored at 2 to 8°C wrapped with Parafilm® to avoid drying.

  4. Pipette out diluted CELLstart™ from culture container and discard. Culture vessel is ready for the addition of cells.

    *Note: It is not necessary to rinse the culture container after removal of CELLstart™. The bottom of the coated culture container should have a clear and wet appearance.

5. Thaw penicillin/streptomycin solution (P/S) overnight at 2 to 8 ℃.

6. Add the above thawed solutions to the Basal Medium, gently swirl the bottle to ensure the mixture homogeneity.

7. Thaw the Human Adipose-derived Stem Cell Supplement (HADSCS) in water bath (37℃) and then add it into the above mixture to make the complete medium. 8. Store the complete medium in the dark at 4℃.

★ Product Use

     This product is for laboratory research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

★ Storage Condition

     Basal Medium should be stored at 4℃, while HADSCS and P/S solution should be stored in the dark at -20℃.