1. Testing Background of Soybean ANFs
A variety of new technologies and methods for eliminating the ANFs （antinutritional factors）in the soybean and its by-products have been developed in the market, such as puffing, expansion, enzymatic hydrolysis, microbial fermentation etc., with the improvement of soybean processing technology. However, the degree of damage to ANFs varies greatly according to the technological level of various methods which is also the main factor for determining product quality. Therefore, it is very important to establish a rapid and accurate testing method to judge the quality of soybean processed products.
Presently the main testing methods for Soybean ANFs are SDS-PAGE, HPLC and HPLC-MS, However, the testing methods based on liquid phase and electrophoresis are not user-friendly in terms of operability, testing cost and testing efficiency, we originally developed the ANFs testing method based on ELISA platform which is combined the production, learning and research mode of the National Feed Center.
2. Special Test Terms of Soybean ANFs based on ELISA Platform
| Glycinin (11S) |
β - conglycinin (7S) |
KTI |
SBA |
3. Product Advantages
3.1 Core technology
We are the first to make ELISA test technology of soybean ANFs be commercialized in China and it has been certified by many authoritative institutions
3.2 Accurate Quantification
Five calibrators with different concentration gradients are calibrated at the same time to ensure accurate quantification
3.3 High Sensitivity
The minimum test limit is lower than the national standard, and the test range is wide to ensure more accurate quantitative quality control.
3.4 High Stability
Testing repeatability of the sample is high, the recovery rate is more than 90%;
The intra-batch precision is less than 10%.
The storage period of the reagent is more than 6 months in cold storage.
4. Comparison of different ANFs Detection methods
| |
SDS-PAGE |
HPLC/HPLC-MS |
ELISA |
| Specification |
Qualitative |
Quantitative |
Quantitative |
| Test time |
8h |
3h |
70min |
| Test cost |
Low |
High |
Medium |
| Operability |
Complex |
Complex |
Simple |
| Reproducibility |
Low |
High |
High |
| Specificity |
Low |
Low |
Good |
| Sensitivity |
Low |
Higher |
High |
| Accuracy |
Ag activity cannot be distinguished |
Ag activity cannot be distinguished |
Suitable for active Ag test |
5. Test Scope
Soybean and soybean products
Soybean meal and soybean meal products
Fermented soybean and fermented soybean products
Extruded-soybean and Extruded-soybean products
Soybean protein
For more details, please feel free to contact with us by email or phone call
Email: zhuliyuan@xinyubio.com
Mobile: +86-18554033120
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