S16 surfactant/SILWET L7600_General reagent_Biochemical reagents_Products_Shenzhen Braveds Biotech co.,Ltd. 

SILWET L-7600 is a polyoxyleneoxide modified polydimethylsiloxane surfactant that combines the strong hydrophobicity of silicone with the hydrophilicity of polyether. Ultra-low surface tension (as low as 20-25 mN/m) can be achieved in water-based and organic solvent systems. Its unique molecular structure gives it a strong wetting ability (even wetting ultra-hydrophobic surfaces such as PTFE), and has anti-coagulation, anti-hemolysis, antistatic and other properties, is a key functional auxiliary in the development of solid phase whole blood diagnostic reagents.

Action mechanism

Super wettability: Through the siloxane chain segment quickly adsorbed to the hydrophobic interface (such as PTFE, nitrate cellulose film), reduce the contact Angle, promote the uniform spread of liquid.

Anti-red cell interference: Inhibit non-specific binding of red blood cells to solid phase carriers in high erythrocyte specific volume (HCT) samples, eliminating detection biases (such as false coagulation positives).

Non-hemolytic: mild action on the erythrocyte membrane, to avoid hemolysis release hemoglobin interference optical detection (such as colorimetry, fluorescence method).

Assisted penetration and leveling: accelerates the diffusion of reagents in dense substrates (such as blood clots, sticky samples) and improves reaction uniformity.

Antistatic property: Reduce the accumulation of particles (such as latex microspheres) caused by electrostatic adsorption to ensure signal stability.

Application scenario

Whole blood direct detection reagent: add to solid state reaction plate or test strip (0.05%-0.2%) to block red blood cell adhesion and improve detection accuracy (such as CRP, D-dimer).

Microfluidic chip: Pre-coated on hydrophobic microchannels (such as PDMS, PTFE) to optimize whole blood/plasma separation efficiency and reduce cell clogging.

Immunochromatographic strips: Sample pad treatment solution (0.1-0.3%) is added to accelerate the penetration of whole blood samples while inhibiting the retention of red blood cells on the binding pad.

Cell lysate enhancer: In combination with a stain remover (e.g. Triton X-100) (0.05%-0.1%), it enhances mild lysis of blood cells/histiocytes.

Optical detection anti-interference: in the colorimetric/fluorescence detection system (0.05%-0.15%), reduce the scattering interference of blood cell fragments to the light signal.

General use range: 0.05%-1.0% (0.1%-0.3% commonly used in whole blood diagnosis).

Matters needing attention

Compatibility test: Avoid direct contact with strong oxidants (such as high concentrations of ammonium persulfate) or silicone adsorbent materials (such as silica columns).

Long-term stability needs to be demonstrated in fluoropolymer systems such as PTFE. Common problems and solutions

Q&A

The whole blood chromatographic speed is too slow: the concentration of S16 may be insufficient; It is recommended to increase to 0.2%-0.3%, or optimize the porosity of the sample pad.

Detection background residual red blood cells: the possibility that red blood cells were not completely blocked; It is recommended to increase the S16 concentration to 0.3% - 0.5%, or extend the reaction time.

The stability of the reagent decreases: it may interact with the components of the system; It is recommended to replace incompatible components (such as cationic polymers) or adjust the order of addition.

Uneven wetting (PTFE surface) : surface pretreatment may be inadequate; It is recommended to pre-clean the substrate (e.g. plasma treatment) before coating S16.

Note: S16 is a flammable liquid, be careful to stay away from the fire source, do not use the use of direct current spray fire extinguisher, otherwise it will cause the fire spread. It is recommended to use anti-alcohol foam, carbon dioxide chemical dry powder fire extinguishers.

SILWET is a trademark of MOMENTIVE