First, system advantages
1. The real-time change of pH can cause changes in the physiological characteristics of strains, and the pH detection function provides strong evidence for metabolic flow analysis
2. The combination of carefully designed micro-feed technology, real-time pH detection and flow-adding technology is of great significance to achieve dynamic research with metabolic flow as the core
3, the control operation of multi-channel and multiple combinations of processes can greatly shorten the process research progress
4, at the same time can be CO2, O2, N2, ventilation, a variety of culture mode; Optionally detect DO;
5, can collect and analyze the exhaust gas;
Ii. Configuration
1, Channel: 8 channels (optional 12/24/36/72 channels)
2, pH system: imported online pH sensor 8, 8 acid pumps, 8 alkali pumps (micro pump, accuracy 0.02ml)
3, feeding system: the use of variable speed pump, can be index, acceleration and other feeding mode, can be measured, cumulative, clear data; After changing the silicone tube with different diameter, it can be re-calibrated and measured. At the same time, add the feed 2 function, can be upgraded to add a feed system;
4, ventilation system: air (O2), N2, CO2, etc., 16 high-precision rotameters
5, temperature system: 10℃-50℃, accuracy: ±0.2℃
6, speed system: 0-400±5 RPM
Third, system functions
1. Data display and record:
Display and record fermentation process parameters such as culture time, temperature, pH, rotational speed, feed amount, acid and base dose, including real-time dynamic curves:
2. Control mode and function:
a, manual control mode: you can set the valve opening ratio or valve opening
b, automatic control mode: can choose PID continuous or PID switch mode control
c, sequence control mode: all control parameters can be pre-set 20 control segments to achieve automatic segmentation control
d, correlation control: pH can be associated with feed control
e, measurement function: It can measure the feed, acid and alkali
f, curve function: can display, analyze real-time dynamic curve, record historical data curve, and can be printed directly
h, calibration function: software to correct pH, peristaltic pump flow rate and other parameters, fast and convenient
i, simulation flow diagram: flow diagram diagram, and display the dynamic real-time data of each detection point in the same interface, intuitive and clear
j, process information query: complete record of each batch of fermentation data and description, you can query historical fermentation data and historical process information description at any time
Fourth, system application
1. Study on pH change rule, inoculation amount and timing of seed transfer during cell growth
2. Optimization of basic media
3. Study on the optimum pH in the growth stage and production stage of bacteria
4. Study on the flow feed, including the type, rate and dynamics of feed
5. Dynamic research with metabolic flow as the core can be realized.