Description
This product uses a unique lysis buffer system to quickly release RNA from cultured cell samples for RT-qPCR reactions, eliminating the time-consuming and laborious RNA purification process, and only 7 minutes to obtain the required RNA template, with the 5× Direct RT Mix, 2× Direct qPCR Mix-Taqman provided by the kit can quickly and efficiently obtain real-time quantitative PCR results.
5× Direct RT Mix and 2× Direct qPCR Mix-Taqman have strong inhibitor tolerance and can perform efficient reversal and specific amplification using the lysate of the sample to be measured as a template. The reagent contains Foregene Reverse Transcriptase, Hot D-Taq DNA Polymerase, dNTPs, MgCl2, Reaction Buffer, PCR Optimizer and Stabilizer, which can be used with lysis buffer to quickly and easily detect samples, and has the characteristics of high sensitivity, specificity and stability.
Specification
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QuickEasyTM Cell Direct RT-qPCR Kit-Taqman
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Kit components
20μl qPCR Reaction System
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DRT-01021
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DRT-01022
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Note
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200 T
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1000 T
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Part I
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Buffer CL
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4 ml
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20 ml
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Cell Lysis
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Foregene Protease Plus II
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80 μl
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400 μl
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Buffer ST
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400 μl
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1 ml × 2
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Part II
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DNA Eraser
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80 μl
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400 μl
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5×Direct RT Mix *
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160 μl
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800 μl
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RT
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2× Direct qPCR Mix-Taqman*
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1 ml × 2
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1.7 ml × 6
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qPCR
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20×ROX Reference Dye
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40 μl
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200 μl
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RNase-Free ddH2O
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1.7 ml
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10 ml
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Instruction Manual
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1 piece
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1 piece
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*:Cell Lysis, 5×Direct RT Mix, 2× Direct qPCR Mix-Taqman can be purchased separately, details are provided in Appendix 1 (PAGE 13).
Product features
- Simple, effective Cell Direct RT technology that takes as little as 7 minutes to get RNA samples.
- Sample requirements are small, and a minimum of 10 cultured cells can be used for experimentation.
- High throughput for rapid RNA acquisition of cultured cells such as 384, 96, 24, 12, and 6-well plates.
- DNA Eraser is able to quickly remove released genomes, greatly reducing the impact on subsequent experimental results.
- Optimized RT and qPCR systems enable two-step RT-PCR with more efficient reverse transcription, specificity, and stronger RT-qPCR reaction inhibitor tolerance.
Kit application
- Scope of application: Cultured cells.
- Sample lysis interpreted RNA: used only as a two-step RT-qPCR template.
- Kits can be used for the following purposes: gene regulatory expression analysis, allele testing, drug screening, etc.
Storage conditions
1. Shipping Conditions
The whole process of low temperature ice pack box transportation, to ensure that the kit is in the <4 °C state.
2. Storage conditions
Store part I at 4°C and Part II at -20°C.
The Foregene Protease Plus II should be stored at 4°C,not frozen at -20°C.
Reagent 2× Direct qPCR Mix-Taqman is stored at -20°C, or at 4°C for short-term use if used frequently (within 10 days).
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