Description

This kit uses the spin column and formula developed by our company, which can efficiently extract small fragments of RNA such as 20-200nt miRNA, siRNA, snRNA from various animal tissues and cells. The kit provides DNA-Cleaning Column, which can easily separate the supernatant from the tissue lysate and remove DNA and long RNA from the sample, which is simple and time-saving; RNA-only Column can efficiently bind RNA with a unique formula , Can process a large number of samples at the same time. The whole system is RNase-Free, so that the purified miRNA is not degraded; Buffer miRW1, Buffer miRW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ions, and organic compound pollution.

Specification

*Please wear gloves and take protective measures during the operation as Buffer RL1 contains irritating chaotropic salts.

Features

◆ The whole process is operated at room temperature (15-25°C), without ice bath and low-temperature centrifugation.

◆ Full set kit RNase-Free, no need to worry about RNA degradation.

◆ DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.

◆ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.

◆ Fast speed: The operation is simple and can be completed within 30 minutes.

◆ Safety: no organic reagent extraction is required.

◆ High quality: The extracted RNA fragments are of high purity, free of protein and other impurities pollution, and can meet the requirements of downstream

◆ Various experimental applications

Kit Application

It is suitable for the extraction and purification of miRNA from a variety of fresh or frozen animal tissues or cells.

Storage conditions

This kit can be stored for 24 months under dry conditions at room temperature (15-25°C), if it needs to be stored for a longer period of time, it can be stored at 2-8°C. Buffer miRL can be placed at 4°C for 1 month after adding β-mercaptoethanol (it is recommended to add it now).

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.