Description
The kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with high polysaccharides or polyphenols content. It provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.
The entire kit does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.
Specification
|
Kit contents
|
RE-0502T
|
RE-05021
|
RE-05024
|
|
5 T
|
50 T
|
200 T
|
|
Buffer PSL1*
|
3 mL
|
25 mL
|
100 mL
|
|
Buffer PS
|
600 μL
|
6 mL
|
24 mL
|
|
Buffer PSL2
|
2.4 mL
|
24 mL
|
96 mL
|
|
Buffer PRW1
|
3 mL
|
25 mL
|
100 mL
|
|
Buffer PRW 2
|
2.4 mL
|
24 mL
|
96 mL
|
|
RNase-Free ddH2O
|
1 mL
|
10 mL
|
40 mL
|
|
RNA-Only Column
|
5
|
50
|
200
|
|
DNA-Cleaning Column
|
5
|
50
|
200
|
|
Manual
|
1
|
1
|
1
|
Features&advantages:
-Particularly suitable for the purification of RNA from plant samples with high polysaccharides or polyphenols content.
-No need to worry about RNA degradation. The whole system is RNase-Free
-Effectively remove DNA using DNA-Cleaning Column
-Remove DNA without adding DNase
-Simple—all operations are completed at room temperature
-Fast—operation can be completed in 30 minutes
-Safe—no organic reagent used
-High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.
Kit application
It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with high polysaccharide or polyphenol content.
Storage and Shelf life
The kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PSL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).
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