The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (mid-atto) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. Moreover, the high stability of UltraScence Femto Plus Western Substrate makes a 2-year- storage at room temperature possible, without any compromise on the performance.
Product Name: UltraScence Femto Plus Western Substrate
Product Item No.: CCH375-B100ML
Size: 100ml
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Store at R.T. for 2 years
US Patent No.: US10,711,185
- No optimization required.
Switching to the UltraScence Femto Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Femto, does not require optimization or protocol changes.
- High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Femto Plus Western Substrate enables an accurate mid femtogram to low femtogram detection of protein on the same immunoblot after a single exposure.
- Optimized for use with PVDF and nitrocellulose membranes.
- Compatible with Western Blotting Markers.
- Optimized for film and CCD-based imaging.
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